Login Pop-up

+ (351) 913908581
Tuesday, January 31, 2023

Fh8 r-PPure System

 

.shadow css class“Fh8 r-PPure System” is a great choice to produce and purify your soluble and active recombinant protein in a simple and cost-effective way. This platform contains a novel fusion partner combining and improving recombinant protein expression, solubilisation and purification. Its low molecular weight, 8 kDa, and its solubility enhancing effect make Fh8 an advantageous option compared to larger fusion tags for soluble protein production in bacteria. Moreover, Fh8 acts has a purification handle due to its affinity to hydrophobic colums.  

A study (Costa et al., 2013) shows that the “Fh8 r-PPure System” is an effective tool for the improvement of protein solubility in E. coli and an excellent candidate for testing expression and solubility next to the other well-known fusion tags. This study was conducted by Portuguese Research Institutions, EMBL at Heidelberg and Hitag Biotechnology.  

Furthermore, in another study conducted by Costa et al, 2013, it was demonstrated that “Fh8 r-PPure System” acts has a purification handle in hydrophobic interaction chromatography and does not interferes with target protein activity.  

Feel free to contact us for additional details or read the following papers: 1) Costa SJ, Coelho E, Franco L, Almeida A, Castro A, Domingues L; The Fh8 tag, a fusion partner for simple and cost-effective protein purification in Escherichia coli; Protein Expression and Purification, in press 2) Costa SJ, Almeida A, Castro A, Domingues L, Besir H; The novel Fh8 and H fusion partners for soluble protein expression in Escherichia coli: a comparison with the traditional gene fusion technology; Applied Microbiology and Biotechnology, 2013, 15:6779-91.

 

Hitag will grant a "non payable" licence for use of “Fh8 r-PPure System” for research purposes only. A separate license is required for any commercial use, including the development of commercial products or services.  Information about commercial licenses may be obtained from Hitag Biotechnology, Lda, Biocant Park L4 N4, 3060-197 Cantanhede, Portugal, email: This email address is being protected from spambots. You need JavaScript enabled to view it.

CONTACT DETAILS

Hitag Biotechnology, Lda
Parque Tecnológico de Cantanhede, N4 L4
3060 -197 Cantanhede, Portugal
E-mail: info@hitag.pt

Map
Scroll to top